De novo genome assembly is a complex task, made more challenging by high levels of genetic diversity, repetitive elements, and duplicated genomic regions. The algorithms to perform these assemblies can be difficult to fine tune for each species and require massive computational resources to weave long-reads into a final, polished assembly. Bringing together multiple technologies (long-read, short-read, next-generation mapping) can improve contigs and scaffolding to provide more complete and accurate genome assemblies.
At DNAnexus we have a team of scientists that provide fast, accurate, and cost-efficient reference quality assembly services. In this webinar we’ll present best practices for PacBio’s FALCON, FALCON-Unzip and NGHRI’s Canu as well as examine the challenges around assembling and phasing the highly repetitive & segmental duplicated regions of Rhesus genome. We’ll also focus on layering next-generation mapping into a de novo assembly providing a genome-wide view to correctly scaffold the sequence data.
DNAnexus is a market leader in de novo genome assembly. Our key experience includes 3000 Rice Genomes Project, Vertebrate Genomes Project, along with many complex individual assemblies, including Rhesus macaque, featured in this webinar. Join us to learn more about de novo assembly best practices.